The Perené River (Spanish: Río Perené) is a Peruvian river on the eastern slopes of the South American Andes.
It is formed at at the confluence of the Chanchamayo River and the Paucartambo rivers, 15 km above the community of Perené, at 650 m above sea level.
The river flows in a south-easterly direction; its length is 165 km.
At the Perené joins the Ene River, 10 km below the community of Puerto Ocopa, at 400 m above sea level, and is called the Tambo River from then on.